Skip to main content
. 2009 Mar 27;191(11):3492–3503. doi: 10.1128/JB.00119-09

FIG. 7.

FIG. 7.

The cupA operon does not contribute to RSCV phenotypes. (A) Diagram of the cupA operon. A downstream gene, PA2133, contains an EAL domain with c-di-GMP-degrading activity. (B) A MJK8 cupA3 insertion mutant containing a resistance cassette from pSP858, MJK8ΔcupA3, does not autoaggregates in liquid culture and does not form a pellicle. When the resistance cassette is excised with FLP recombinase (MJK8ΔcupA3-FLP), autoaggregation and pellicle formation are restored. We also deleted the cupA1 gene in MJK8 and PAO1, which resulted in no observable phenotype. We mutated cupA3 by replacing the gene with a resistance cassette from pSP858, which restored wild-type colony morphology to MJK8 and eliminated autoaggregation in liquid culture. Additionally, in a culture of this mutant, no pellicle was produced. These phenotypes are similar to those of the parent strains harboring an EAL overexpression construct, so we hypothesized that the mutation may have a polar effect on the downstream EAL domain gene, PA2133. When the resistance cassette is excised from cupA3 with FLP recombinase, colony morphology and autoaggregation are restored to the parental MJK8 phenotypes.