TABLE 1.
Bacterial strains and plasmids
| Strain(s) or plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild type | |
| MJK8 | Biofilm-derived rugose strain (isogenic to PAO1) | 21 |
| MJK8ΔcupA3 | cupA3 polar mutant of MJK8 | This study |
| MJK8ΔcupA-FLP | cupA3 nonpolar mutant of MJK8 | This study |
| MJK8Δpslpel | pslBCD pelA mutant of MJK8 | This study |
| MJK8Δ1169 | PA1169 mutant of MJK8 | This study |
| CF37wt to CF39wt | Clinical longitudinal isolates with wild-type phenotype | 6, 21 |
| CF38s and CF39s | Clinical longitudinal isolates with rugose phenotype | 6, 21 |
| CF39sΔpslBCD | pslBCD mutant of CF39s | This study |
| CF39sΔpelA | pelA mutant of CF39s | This study |
| CF39sΔpslBCDΔpelA | pslBCD pelA mutant of CF39s | This study |
| WF801 | PAO1ΔpslBCD expressing PBAD-psl | 1 |
| WF800 | pslBCD mutant of PAO1 expressing PBAD-psl | 23 |
| Plasmids | ||
| pFLP2 | Source of FLP recombinase, Ampr | 18 |
| pEX18.Ap | Suicide cloning vector, Ampr | 18 |
| pSP858 | Source of gentamicin resistance cassette, Ampr Gentr | 18 |
| pSP5 | WspF expresson vector, Ampr | This study |
| pSP6 | PA2133 expression, Ampr | This study |
| pUCP18 | E. coli-P. aeruginosa expression vector, Ampr | 34 |
| pMPSL-KO1 | pslBCD allelic replacement vector | 21 |
| pMPELA | pelA allelic replacement vector | This study |
| pMCUPA3 | cupA3 allelic replacement vector | This study |
| pM1169 | PA1169 allelic replacement vector | This study |