TABLE 2.
Primers used for PCR amplification of the promoter regions and for primer extension reactions
| Oligonucleotide name | Sequence (5′→3′)a |
|---|---|
| A1F | CCTTGGATCCATTATGAGTTTTGACAGCAT |
| A1R | GGGAGAATTCAAGTTTGTAGCGTATTTATT |
| A2F | TTTAGGATCCTTTTGCTCAGTGGTTGTCAC |
| A2R | TATAGAATTCGACGCTGGTTTTGCGATAGC |
| A3Fnew | ATTTGGATCCAGCGTCAAAGAATGTCTTGT |
| A3Rnew | TAATGAATTCTTTCAAGTAGAATCACG |
| A3.1 | GGAATTCACGAACGCCTAT |
| A3.2 | CGGGATCCTATGATAAAATT |
| A3.3 | GGAATTCAATTCGCAGAAT |
| A3.4 | CGGGATCCAAAAGATTTTCA |
| A3.6 | TCCCCCGGGAATAAAATTTTA |
| A3.7 | TCCCCCGGGCCCATGAAAATC |
| A3.8 | TCCCCCGGGTTAATAGGCGTT |
| A3.9 | TCCCCCGGGAAAATAAAAAAA |
| PEfecA1 | CCAAAACAGCCAAAGAGACTA |
| PEfecA2 | ATTTTTGCTCAGTGGTTGTCA |
| 5PEfecA3 | ATTCTTTCAAGTAGAATCACGC |
| A3Z1 | GTATCGATAAGCTTGATATC |
| 5′kat | CACATCTTTATTAACCAT |
a
Bases in italics correspond to exogenous restriction sites.