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. 2009 Mar 25;83(11):5943–5946. doi: 10.1128/JVI.00188-09

FIG. 2.

FIG. 2.

Protein synthesis in DF1 cells infected with recombinant viruses. (A) Western blot of HN and M proteins from parental and HN UTR mutant virus-infected cells. DF1 cells were infected at 5 PFU/cell, and total proteins were collected at 24 h postinfection. Lanes: 1, rBC; 2, rBC-5′UTRΔ1-6; 3, rBC-5′UTRΔ76-81; 4, rBC-5′UTRΔ1-78; 5, rBC-3′UTRΔ1-6; 6, rBC-3′UTRΔ161-166; 7, rBC-3′UTRΔ40-63; 8, rBC-3′UTRΔ1-162; 9, rBC-5′UTRΔ1-78/3′UTRΔ1-162; and 10, rBC-NP5′UTR/NP3′UTR. (B) Ratios of quantified HN protein to M protein levels from the parental virus, rBC, and the HN UTR mutant viruses. Each bar shows the mean and standard error for the mean of triplicate samples.