FIG. 3.

S. sarrachoides stem and leaf tissue immunoblots. Plants were agroinoculated with WT virus or the ΔP5 mutant 3 weeks prior to destructive sampling from many sites on the plant. Samples from similar regions on healthy plants were also collected. Virus antigen is detected as a purplish-blue immunostained region, some of which are indicated by arrowheads. The internal (Int) and external (Ext) phloem bundles are labeled. Immunoblots on tissue collected from plants infected with the SST, QSS, and SYG mutants were also completed (data not shown). The level and distribution of virus for SST and QSS were similar to those of the WT, and SYG was similar to ΔP5.