FIG. 6.

Measurement of virus and P3 protein accumulation in WT-, ΔP5-, and SYG-infected leaves using Western blot analysis and DAS-ELISA. Three leaves each from pruned WT-, ΔP5-, and SYG-infected plants were divided into two halves. (A) The P3 protein was detected in total protein extracts in one half of the leaf by Western blot analysis. (B) Virus accumulation was detected in the other half of the leaf using DAS-ELISA. The mean absorbance values (A₄₀₅) ± standard errors are shown. One-way analysis of variance indicated a significant effect of virus type on antigen accumulation (F = 69.53; df = 2; P < 0.001), and treatment means were all significantly different from each other according to the Newman-Keuls test (P = 0.05).