FIG. 8.

Analysis of SYG revertant-infected leaf tissue. (A) SYG-infected S. sarrachoides leaf exhibiting WT-like symptoms. (B) Enlarged view of a SYG-infected whole-leaf tissue immunoblot from the same leaf showing virus confined to phloem tissue. (C) SYG revertant sequences (sequences SYG-1 to -4) obtained from progeny virus in four different infected leaves. WT and SYG sequences are shown for comparison. In the SYG sequence, the codon containing a deleted nucleotide (14 nt downstream from the SYG deletion) that resulted in a frameshift mutation is underlined. Amino acids and nucleotides that were altered in the pseudorevertants are shown in red type. Sequences SYG-1 to -4 originate from leaves manifesting WT-like symptoms.