FIG. 9.

RT-PCR to detect the presence of virus in aphids after 24- and 72-h periods of feeding on virus-infected tissue to determine if aphids ingested virus from infected leaves. Primers amplify a 624-bp fragment of the P3 ORF. Primers that amplify a 301-bp fragment of the 18S rRNA were used as an RNA control. Aphids were fed on tissue systemically infected with WT, QSS, ΔP5, or SYG virus. WT and QSS viruses were identical, with the WT being the representative sample shown. Two groups of three aphids were collected at each time point for each sample.