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Representative heparin-Sepharose NaCl elution profiles. 35S-labeled viruses were grown in BHK or C7/10 cells, gradient purified, and applied to heparin-Sepharose columns. Elution was performed with a 100 mM-to-500 mM NaCl gradient. Fractions were collected and analyzed for 35S cpm and [NaCl] to determine the NaCl concentration at which there was peak virus elution.
