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Virus replication in C7/10 mosquito cells and in Culex quinquefasciatus. Cells were infected at an MOI of 10 with TE12 or an E1-139 or E1-245 mutant (A) or TE12 or an E2-196 or E2-318 mutant (B) and monitored for production of infectious virus by plaque formation in BHK cells. Mosquitoes were inoculated intrathoracically with 100 PFU of TE12 or an E1-139 or E1-245 mutant (C) or TE12 or an E2-196 or E2-318 mutant (D) and monitored daily for virus replication.
