FIG. 4.

Impacts of ERM proteins on NS1 phosphorylation. A9 cells or derivatives thereof were infected with MVM (30 PFU/cell), metabolically labeled 24 h p.i., and processed further for determination of the NS1 phosphorylation pattern by two-dimensional tryptic phosphopeptide analysis. The dotted ovals mark peptides whose phosphorylation is PKCη dependent. White ovals, phosphorylation pattern of NS1 produced in parental A9 cells; black ovals, patterns lacking specific phosphopeptides; black arrow, additional phosphopeptide. PKCη-TA, PKCηT512A.