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. 2009 Mar 11;83(11):5574–5580. doi: 10.1128/JVI.00001-09

FIG. 2.

FIG. 2.

In vitro regulation of EGFP expression from AAV and IDLV by tTRKRAB. 293T cells carrying 20 copies of LV-tTR-KRAB-Red were transduced in the presence or absence of Dox with either rAAV encoding mPGK-eGFP-tetO at a multiplicity of infection (MOI) of 1.5 or LV-pFUT or IDLV at an MOI of 30 or 40, respectively. 293T cells containing no LV-tTR-KRAB-Red vector were transduced under the same conditions in the presence of Dox. The cells were FACS analyzed at 48 h posttransduction. For the LV-tTR-KRAB-Red-transduced cells, the percentage of EGFP-positive cells is gated on the dsRed-positive population.