FIG. 8.
Analysis of the 3′ termini of EAV and SARS-CoV NendoU products. (A) Proposed mechanism for the transphosphorylation (1) and hydrolysis (2) reactions catalyzed by RNase A. (B) Cleavage products obtained after incubation of 1 μM unlabeled RNAsGUA with 0.6 μM EAV nsp11 or SARS-CoV nsp15 for 2 min (1) or 40 min (2) at 30°C under standard reaction conditions were subsequently ligated to 5′-[32P]pCp without further treatment (lanes a) or after dephosphorylation with T4 PNK (lanes b) or CIAP (lanes c). The reaction products were analyzed by denaturing PAGE and phosphorimager analysis. The full-length substrate (*) and the 5′ cleavage product (▴) are indicated.