FIG. 1.

Formation of viral transcriptosomes requires de novo protein synthesis. G₀-synchronized cells were released into G₁, infected with HCMV Towne (MOI of 5), and seeded onto glass coverslips. At 30 min p.i.,100 μg/ml CHX or control medium was added. Mock infections were performed under the same conditions. At 8 h p.i., cells were washed with PBS, fixed with 2% FA, permeabilized, and stained with antibodies specific for pp65 (A) and for IE1/2 and cdk9 (B). Fluorescein isothiocyanate- and Cy3-conjugated isotype-specific secondary antibodies were used. Nuclei were stained with Hoechst dye. White arrows show examples of viral transcriptosomes, although more are present. All of the images represent confocal optical 0.2-μm sections at a magnification of ×1,000 under conditions of oil immersion.