FIG. 4.

Ellagic acid and spiperone cause concentration-dependent inhibition of SV40 but not adenovirus. (A) In the left panels, mock-infected or SV40-infected HeLa-E6 cells were treated with the indicated compounds for 2 days. The cells were then harvested, stained for large T antigen expression, and subjected to flow cytometry. In the right panels, HeLa-E6 cells were mock infected or infected with a recombinant adenovirus expressing GFP and then treated with the indicated compounds for 2 days. The cells were then harvested and subjected to flow cytometry to quantify GFP expression as a marker for adenovirus infection. (B) HeLa-E6 cells were infected with SV40 and treated with various concentrations of ellagic acid (dashed line) and spiperone (solid line). Large T antigen positive cells were enumerated by flow cytometry, corrected for multiple viral infections per cell by using the Poisson distribution, and expressed as the percentage of T antigen positive cells in the absence of compound. Solid line, spiperone; dashed line, ellagic acid. In the absence of test compound, ca. 60% of infected cells were large T antigen positive in this experiment. The experiment shown is representative of five independent experiments with similar results.