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Ellagic acid and spiperone inhibit the expression of SV40 T antigen RNA. SV40-infected HeLa-E6 cells were treated with the indicated concentrations of ellagic acid and spiperone, and total RNA was prepared 2 days later. T antigen and GAPDH mRNA were quantified by using qRT-PCR. The GAPDH mRNA levels were used to normalize the signal between different samples. The relative levels of T antigen mRNA in treated cells is expressed as the percentage of the amount of mRNA in untreated cells.
