FIG. 4.

rJUNV-Furin exhibits altered virus growth and glycoprotein maturation. (A) Vero E6 cells were infected with wt JUNV (triangle), rJUNV (square) and rJUNV-Furin viruses (circle). Duplicate samples were collected and titers determined at 0, 1, 3, 5, and 7 days p.i. (B) Protein lysates from Vero infected cells were examined by Western blot using anti-JUNV GPC MAbs. Glycosylated forms of GPC and G2 forms are indicated with open arrows and unglycosylated forms with black arrows. EndoH resistant forms of GPC (asterisk), and G2 (triangle and circle) are also indicated. Blot was reprobed with anti-actin MAb to control the amount of loaded proteins in the gel.