FIG. 5.

rJUNV-Furin grows in SKI-1/S1P deficient cells. SRD12B cells deficient in SKI-1/S1P were infected with rJUNV or rJUNV-Furin in the absence or presence of 30 μM the furin inhibitor dec-RVKR-CMK. Thirty-six hours p.i. supernatants were replaced with fresh media with or without inhibitor. JUNV proteins were detected 3 dpi using an anti-Junin rabbit serum and an anti-rabbit Alexa Fluor 546 (red stain). Cells were counterstained with DAPI and photographed with UV light in a regular upright microscope.