FIG. 4.
(A and B) Immunoblots of electrophoretically separated lysates from Vero cells infected with wild-type HSV-1(F). Infected Vero cells were harvested at the indicated times postinfection and analyzed by immunoblotting with anti-Us3-S147P antibody (A) or anti-Us3 antibody (B). (C and D) Amount of Us3-S147P protein shown in panel A (C) and total Us3 protein shown in panel B (D) as a function of time postinfection, relative to the amounts at 12 h postinfection. The data were normalized to the values at 12 h postinfection. α, anti; ND, not detected; Total Us3, Us3 protein detected by anti-Us3 polyclonal antibody.