FIG. 3.

UL138 amino acid sequence and recombinant UL138 viruses. (A) Amino acid sequence of pUL138. The putative transmembrane domain is shaded, and the immunizing peptide used to create the rabbit anti-UL138 antibody is underlined. (B) Schematic of the recombinant viruses FIXsubUL138, FIX-UL138_STOP, and FIX-UL138_myc. (C) Multistep growth curve analysis of FIX recombinant viruses. MRC5 cells were infected at 0.01 TCID₅₀ per cell and virus titers measured by TCID₅₀ over the time course indicated. Values represent the averages from two independent experiments.