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The UL138 transcripts synthesized in cells infected with FIX-UL138STOP are stable. Cells infected with FIXwt (FIX) or FIX-UL138STOP (STOP) at an MOI of 2 were treated with 4 μg/ml actinomycin D at 16 hpi. Total RNA was isolated over a time course and analyzed by Northern blotting using a radiolabeled antisense probe to UL138. 18S and 28S rRNAs for each blot are stained with methylene blue and served as a loading control (lower two panels).
