TABLE 2.
Primary antibodies used for immunofluorescence and immunoblotting
| Antigena | Antibody | Source | Typeb | Dilution
|
|
|---|---|---|---|---|---|
| Immunofluorescencec | Immunoblottingd | ||||
| UL138 | 5484, custom | Open Biosystems | R | 1:250 | 1:1,000 |
| Myc epitope | Clone 9E10 | Millipore | M | 1:1500 | 1:5,000 |
| Myc epitope | 71D10 | Cell Signaling | R | 1:200 | NDe |
| MHC-I, HLA-B/C | HC10 | Giftf | M | ND | 1:50 |
| IE1/2 | 3H4 | Giftg | M | ND | 1:100 |
| pp71 | 2H10-9 | Giftg | M | ND | 1:100 |
| α-Tubulin | DM 1A | Sigma | M | ND | 1:12,000 |
| β-Actin | A2103 | Sigma | M | ND | 1:1,000 |
| Golgi GM130 | Clone 35 | BD Transduction Laboratories | M | 1:100 | ND |
| Golgi G58K | Clone 58K-9 | Gene Tex | M | 1:125 | ND |
| GS 27 | Clone 25 | BD Transduction Laboratories | M | 1:100 | ND |
| PDI | RL 77 | Affinity Bioreagents | M | 1:50 | ND |
| EEA1 | 14/EEA1 | BD Transduction Laboratories | M | 1:250 | ND |
a
GM, Golgi matrix; GS, Golgi SNARE.
b
R, rabbit; M, mouse monoclonal.
c
Dilution in PBS supplemented with BSA and Tween 20; incubated for 60 to 90 min at room temperature or at 4°C overnight in the case of the rabbit anti-Myc antibody.
d
Dilution in TBS supplemented with BSA and Tween 20; incubated for 60 to 90 min at room temperature.
e
ND, not done.
f
Provided by Lonnie Lybarger (51).
g
Generous gift from Tom Shenk, Princeton University.