FIG. 4.

ICP27 arginine-to-lysine viral mutants exhibit a growth defect. One-step viral growth curves were performed with the ICP27 R-to-K mutants. (A and B) Vero cells were infected with wild-type (WT) HSV-1 KOS, the ICP27 null mutant 27-GFP, ΔRGG, or the ICP27 R-to-K mutants at a MOI of 1. Infected cells were harvested 0, 4, 8, and 16 h after infection. Plaque assays were performed on Vero cells. Plotted is the average titer (n = 3). (C) As described in Materials and Methods, viral mutants were rescued. Average titers are plotted (n = 3). (D) The average plaque areas of WT HSV-1 KOS virus and viral mutants were measured. Error bars represent the standard errors of the means (n = 10).