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In vitro replication of DkUkr63-Wt in comparison with the HACS mutants DkUkr63-It98HACS, DkUkr63-Hk220HACS, and DkUkr63-R28HACS. (A) Plaque assays on MDCK cells in the presence and in the absence of trypsin. (B) Western blots of supernatants from infected MDCK cells incubated in the presence (T) and in the absence (−) of trypsin, using a monoclonal antibody specific to an HA of the H3 serotype.
