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. 2009 Mar 25;83(11):5869–5880. doi: 10.1128/JVI.01821-08

FIG. 4.

FIG. 4.

FIG. 4.

BC3 and BCBL-1 cell transactivation of mRNAs is inhibited in the absence of K-bZIP. TREx BCBL-1-shRNA-K8 cells were stimulated with or without 1 μg/ml doxycycline for 72 h in the presence of TPA-BAc. Cells were harvested at 0, 4, 24, 48, and 72 h. BC3-shRNA-K8 or BC3-shRNA-ctl cells were stimulated with TPA-BAc. Cells were harvested at 0, 6, 24, 48, and 72 h. For each time point, total RNA was isolated and processed for ORFK8 (A), ORF50 (B), ORF57 (C), and ORF26 (D) by RT-QPCR. (E to G) TREx BCBL-1-shRNA-K8 cells were stimulated with or without 1 μg/ml doxycycline for 24 h in the presence of TPA (20 ng/ml) and BAc (0.3 mM). Cells were harvested at 0, 2, 4, 6, and 8 h. For each time point, total RNA was isolated and processed for ORFK8 (E), ORF50 (F), and ORF57 (G) RT-QPCR. Results are expressed as mean (triplicate) inductions (n-fold) ± SD relative to nonstimulated cells after normalization of samples with GAPDH expression. Results are representative of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.