FIG. 6.

Autophagy is induced in MRC-5 or MeWo cells infected with cell-free preparations of VZV vOka/Merck. (A) Replicate cultures of MRC-5 cells were mock infected or infected with inoculum consisting of either trypsin-dispersed (CA) or sonically disrupted (CF) infected MRC-5 cultures. Inoculum preparations had been titrated in advance, and the multiplicity of infection was approximately 0.02 PFU/cell. At different time points after infection, cells were harvested, solubilized, and subjected to immunoblotting with antibodies against β-actin, LC3B, and viral gE. (B) Replicate cultures of MeWo cells were either mock infected or infected with a virus inoculum consisting of cell-free VZV vOka/Merck at a multiplicity of infection of approximately 0.01 PFU/cell. At various time points after infection, cells were harvested, solubilized, and subjected to immunoblotting with antibodies against LC3B and viral gE.