FIG. 5.

Other unaffected properties of D-fraction cores. Cores of MRV strain T1L were subjected to 1-h transcriptions with either no DMSO or 6% DMSO. Representative results are shown from a total of two experiments in each case. (A) Mg²⁺ dependence. Variations relative to standard Mg²⁺ concentration (9.5 mM, shown as 0 on the x axis) were achieved by adding either EDTA or additional MgCl₂ to the standard transcription mix. Transcription yields are expressed as a percentage relative to that with no DMSO and standard Mg²⁺ concentration. (B) Spermidine sensitivity. Transcription yields are expressed as a percentage relative to that with no DMSO and no spermidine.