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. 2009 Mar 25;83(11):5881–5889. doi: 10.1128/JVI.02345-08

FIG. 6.

FIG. 6.

Phenotypic analysis of vaccine-induced CD8+ T cells. The memory phenotype of antigen-specific T cells was determined in 10 animals (5 animals from each primed group). PBMC were unstimulated (Neg) or stimulated with Env peptides for 16 h and stained with IFN-γ, IL-2, TNF-α, CD3, CD4, CD8, CCR7, and CD45RA antibodies. A flow cytometry scatter plot of CD8+ T cells from animal Ri508 (DNA primed) at week 26 (peak) is shown. The red dots indicate the cytokine-producing vaccine-induced CD8+ T cells. Env-specific CD8+ T cells are of the EM (CD45RA− CCR7−) and EFF (CD45RA+ CCR7−) phenotypes.