FIG. 7.
Loss of PRMT1 in MEFs leads to genomic instability by SKY analysis. SKY was performed on the PRMT1FL/−;CreERT MEF cells untreated (-OHT) or treated with OHT for 6 days (+OHT). SKY was carried out as described in Materials and Methods. A summary of all chromosomal abnormalities for each cell line is given in Table 1. Two representative metaphase spreads of the +OHT cell line analyzed by SKY are shown. (A) Spectral karyotype of a metaphase with 142 chromosomes, represented in classification pseudocolors. (B) Spectral karyotype of a metaphase with 71 chromosomes. The karyotype is classified with the inverted-DAPI image (left) and the classification pseudocolors (right). Arrows indicate the chromosomal abnormalities that are found only in the +OHT cell line, including unique chromosome deletions and translocations, chromosome breaks, and dicentric chromosomes. The inverted-DAPI image of this metaphase is shown in Fig. S2 in the supplemental material.