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. 1999 Mar 2;96(5):2538–2542. doi: 10.1073/pnas.96.5.2538

Figure 3.

Figure 3

TonEBP[1–472] inhibits stimulation of transcription by hypertonicity. (Left) MDCK cells were transiently transfected with 2 μg of a plasmid containing a TonE-driven luciferase gene (15) along with plasmid pcDNA3.1(+) (Invitrogen) directing expression of TonEBP[1–472] or pcDNA3.1(+) by itself (vector) in amounts indicated at the bottom. Transfected cells were cultured in isotonic (open bars) or hypertonic medium (filled bars) for 18 hours, and the luciferase activity was measured. Luciferase activity was normalized to the control cells [transfected with the pcDNA3.1(+) and the luciferase construct] cultured in isotonic medium. Results are mean ± SEM; n = 4. (Right) Lack of effect of TonEBP[1–472] on tumor necrosis factor α-induced NF-κB activity. MDCK cells were transfected with 2 μg of a plasmid containing the luciferase gene under the control of κB sequence from the immunoglobin κ light chain gene (19) along with 3 μg of TonEBP[1–472] in pcDNA3.1(+) or pcDNA3.1(+) by itself as indicated at the bottom. The transfected cells were treated without or with tumor necrosis factor α (20 ng/ml) for 6 hours and were analyzed for luciferase activity. Fold-induction of luciferase by tumor necrosis factor α treatment is shown. Results are mean ± SEM; n = 4.