FIG. 10.

Dysregulation of NRG1 and ErbB4 expression patterns in the developing forebrain of Celsr3 mutants. NRG1 immunostaining of the E15.5 control (A) and mutant (A′) neocortex, counterstained with DAPI. NRG1-expressing strip is much thinner in mutants (A′) than in controls (A; highlighted in inset). The expression of ErbB4 is increased in the mutant corticostriatal boundary (B, B′, C, and C′). The total amounts of NRG1 protein are similar in mutants and controls (D and E) (P = 0.81 [n = 6] with a two-tailed Student's t test). The expression patterns of Sema3A, Sema3F, Npn1, and Npn2 are the same in mutants and controls (F to J and F′ to J′). CP, cortical plate; Ctx, cortex; Hip, hippocampus; IZ, intermediate zone; LV, lateral ventricle; PCx, piriform cortex; Str, striatum. Bar in panel B′, 650 μm for panels A and A′, 1,000 μm for panels B and B′, and 125 μm for panels C and C′; bar in panel J′, 1,000 μm for panels F to J and F′ to J′.