FIG. 2.
TTK/hMps1 interacts with p53 in vitro and in vivo after spindle disruption or DNA damage in 293T cells. 293T cells were treated with Taxol (100 nM) (A), IR (8 Gy) (B), or UV (30 J/m2) (C) and then collected at the indicated times. TTK was immunoprecipitated from these cells using an anti-TTK antibody, and p53 in the immune complex was detected by Western blotting using the anti-p53 antibody PAb1801. (D and E) p53 C terminus interacted directly with the kinase domain of TTK/hMps1 in vitro. Recombinant His-tagged p53 or the truncation mutants ΔN and ΔC (D) were mixed with GST-fused full-length or truncated TTK (E) in GST pulldown assays. Bound proteins were detected by Western blotting.