TABLE 2.
Translesion synthesis events in wild-type and Rev1-deficient MEFs
| Nucleotide(s) inserted opposite lesiona | No. (%) of colonies with events per genotype at UV lesion:
|
|||
|---|---|---|---|---|
| (6-4)PP-TT
|
CPD-TT
|
|||
| Wild type | Rev1−/− | Wild type | Rev1−/− | |
| C-AA-C | 8 (17) | 24 (55) | 41 (87) | 33 (85) |
| A-AA-C | 1 (2) | 2 (4) | ||
| G-AA-C | 1 (2) | |||
| C-TA-C | 1 (2) | |||
| C-AC-C | 1 (2) | |||
| C-AG-C | 2 (4) | |||
| C-AT-C | 2 (4) | 2 (5) | ||
| C-AA-A | 20 (43) | 4 (9) | ||
| C-AA-G | 1 (2) | |||
| C-AA-T | 2 (5) | |||
| Δ-AA-C | 1 (2) | |||
| C-AΔ-C | 2 (4) | 1 (2) | ||
| C-GT-C | 1 (2) | |||
| C-AC-A | 1 (2) | |||
| C-AT-A | 1 (2) | |||
| C-GA-A | 4 (9) | 2 (5) | ||
| C-TA-A | 3 (6) | 1 (2) | ||
| A-AA-A | 1 (2) | |||
| Overall totalb | 47 (100) | 44 (100) | 47 (100) | 39 (100) |
| Total mutagenic | 39 (83) | 14 (32) | 4 (9) | |
a
Inserted nucleotides are in boldface. Δ indicates one nucleotide deletion.
b
Numbers of colonies with deletions/insertions were as follows: for (6-4)PP-TT in Rev1−/− cells, 6 (14%); for CPD-TT in wild-type and Rev1−/− cells, 2 (4%) and 6 (15%), respectively.