FIG. 8.

Model for Plk1 relocalization during mitosis and effect of centrosomally tethered Plk1 on mitotic progression. (A) Model for Plk1 localization and dynamics during normal mitosis. In prophase and prometaphase, Plk1 kinase activity facilitates its release from the centrosome and promotes its localization to kinetochores, the spindle midzone and the midbody through self-priming. Some residual Plk1 localization on kinetochores remains during telophase. (B) Model for the effect of centrosomally tethered Plk1 (Plk1-AKAP) during mitosis. Plk1 tethering to centrosomes induces spindle checkpoint activation and mitotic arrest, indicating that Plk1 dissociation from centrosome is important for further mitotic progression. (C) Centrosomally tethered Plk1 (Plk1-AKAP) (bottom, thin arrow) is less effective than fully dynamic WT Plk1 (top, thick arrow) at stimulating mitotic reentry after DNA damage.