TABLE 1.
Dynamics of Plk1 at different subcellular locations
| Location | Plk1 | Mean 50% recovery time (s) ± SEM | Mean net fluorescence recovery intensity (%) ± SEM | No. of independent expts |
|---|---|---|---|---|
| G2 centrosomes | WT | 17.9 ± 2.2 | 48.3 ± 4.1 | 14 |
| T210D | 19.0 ± 3.5 | 52.1 ± 4.4 | 9 | |
| D176N | 16.6 ± 4.2 | 65.8 ± 8.9 | 6 | |
| T210A | 14.7 ± 2.0 | 42.6 ± 9.1 | 14 | |
| PBD | 25.8 ± 1.8 | 20.4 ± 5.8 | 15 | |
| WT-AKAP | 58.2 ± 6.8 | 31.7 ± 3.6 | 11 | |
| Mitotic centrosomes | WT | 7.0 ± 1.3 | 28.3 ± 2.0 | 7 |
| T210D | 6.9 ± 0.7 | 30.3 ± 4.6 | 6 | |
| D176N | 10.0 ± 0.8 | 21.8 ± 1.8 | 6 | |
| T210A | 9.3 ± 2.2 | 31.8 ± 4.4 | 8 | |
| PBD | 16.7 ± 1.0 | 17.4 ± 1.8 | 19 | |
| Kinetochores | WT | 12.4 ± 1.3 | 29.1 ± 2.5 | 17 |
| D176N | 9.4 ± 0.8 | 23.2 ± 1.5 | 14 | |
| PBD | 15.0 ± 1.5 | 17.5 ± 2.0 | 12 | |
| Midbodies | WT | 65.7 ± 10.0 | 23.3 ± 5.5 | 12 |
| T210D | 75.4 ± 10.9 | 31.0 ± 4.6 | 5 | |
| D176N | 8.3 ± 4.2 | 17.9 ± 3.1 | 8 | |
| T210A | 67.8 ± 19.5 | 31.2 ± 6.0 | 6 | |
| PBD | 108.1 ± 9.8 | 12.7 ± 1.3 | 9 |