FIG. 5.

Input to trigger Cdc42 activity is derived from both β1 and β2 integrins in T cells. (A) The β1-integrin-deficient A1 cells and A1-derived lines that have been reconstituted with the WT or mutant β1 integrin constructs were transfected with Raichu-Cdc42 (or the T17N mutant) and adhered on anti-CD43 (left) or anti-LFA-1 (right) antibody-coated coverslips for 30 min at 37°C. (B) Bar charts represent the cumulative FLIM/FRET data of three experiments (n = 7 cells). FRET/FLIM analysis as described for Fig. 1. **, P < 0.005 by t test. Scale bars = 5 μm.