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. 2009 Mar 30;29(11):3033–3044. doi: 10.1128/MCB.01449-08

FIG. 7.

FIG. 7.

Loss of RGS9-2 but not R7BP induces changes in RGS9 localization in striatal neurons. Electron micrographs showing immunoreactivity for RGS7 in wild-type (WT), RGS9 knockout (RGS9 KO), and R7BP knockout (R7BP KO) mice in the striatum, as revealed using pre-embedding (A, B, D, E, G, and H) and postembedding (C and F) immunogold techniques. Using the pre-embedding immunogold method, immunoparticles for RGS7 in wild-type neurons (A and B) were mainly located at intracellular sites (e.g., crossed arrows), associated with intracellular membranes within dendritic shafts (Den) and spines (s) establishing excitatory synapses with axon terminals (b). In less proportion, immunoparticles for RGS7 were also detected along the plasma membrane (e.g., arrows). Using the postembedding immunogold method (C), immunoparticles for RGS7 in wild-type neurons were mainly located at intracellular sites (e.g., crossed arrows) and occasionally along the postsynaptic density of excitatory synapses (e.g., arrowheads). Using the pre-embedding immunogold method, immunoparticles for RGS7 in RGS9 KO neurons (D and E) were mainly located along the plasma membrane (e.g., arrows) of dendritic shafts (Den) and spines (s) establishing excitatory synapses with axon terminals (b). In less proportion, immunoparticles for RGS7 were also detected at intracellular sites (e.g., crossed arrows), associated with intracellular membranes. Using the postembedding immunogold method (F), immunoparticles for RGS7 in the RGS9 KO were mainly located along the postsynaptic density of excitatory synapses (e.g., arrowheads) and occasionally at intracellular sites (e.g., crossed arrows). RGS7 immunoreactivity in R7BP KO neurons (G and H) replicated the pattern observed in wild-type samples. That is, immunoparticles for RGS7 in the R7BP KO were mainly located along the plasma membrane (e.g., arrows) of dendritic shafts (Den) and spines (s), establishing excitatory synapses with axon terminals (b). In less proportion, immunoparticles for RGS7 were also detected at intracellular sites (e.g., crossed arrows), associated with intracellular membranes. Scale bar: A, B, D, E, G, and H, 0.5 μm; C and F, 0.2 μm.