TABLE 3.
Oligonucleotides used in this study
| Name | Sequence (5′ to 3′)a |
|---|---|
| 3HASalI-5′ | GATGATGTCGACTCTAGATACCCATAC |
| 3HAXhoI-3′ | GACCTCGAGCTAGCTAGTTCTAGAAGC |
| Rcn1PstI-5′ | GCCCTGCAGATGGGTAATATTATAACGGAT |
| Rcn1(1-196)SalI-3′ | GATGATGTCGACTTTAACGTGGTCCGCTAA |
| Rcn1(1-172)SalI-3′ | GATGATGTCGACCACCTTTGATTTCAATAA |
| Rcn1(+99)PstI-5′ | GCCCTGCAGATGCAATACTTGAAGGTA |
| Rcn1(+120)PstI-5′ | GCCCTGCAGATGCCCGAATTCGATTTC |
| Rcn1SalI-3′ | GATGATGTCGACATCATCGTCATC |
| Rcn2PstI-5′ | GCCCTGCAGATGGCAAACCAAAAGCAAATG |
| Rcn2SalI-3′ | GATGATGTCGACATGGAAAAACTCGTTAAC |
| Rcn2(1-255)SalI-3′ | GATGATGTCGACACTTTTTGGAGGATTTGA |
| Rcn2(+86)PstI-5′ | GCCCTGCAGATGAAGTTATTTGTAACTGAG |
| RCAN1PstI-5′ | GCCCTGCAGATGCATTTTAGAAACTTTAAC |
| RCAN1SalI-3′ | GATGATGTCGACGCTGAGGTGGATCGGCGT |
| RCAN1(1-153)SalI-3′ | GATGATGTCGACAGTGGTGTCAGTCGCTGC |
| RCAN1(1-180)SalI-3′ | GATGATGTCGACCTTAGGTCTCCTCATTCTTTC |
| RCAN1(+94)PstI-5′ | GCCCTGCAGATGTCACACCTGGCTCCG |
| RCAN1(+115)PstI-5′ | GCCCTGCAGATGGTGGGATGGAAACAAGTG |
| RCAN1(S108A)F | GCAGTTCCTGATCGCCCCTCCCGCCTCTC |
| RCAN1(S108A)R | GAGAGCCGGGAGGGGCGATCAGAAACTGC |
| Rcn1(AxA)F | CAAAGGTGGGCGCAGCAACCGCAGATAGATGTCCCAC |
| Rcn1(AxA)R | GTGGGACATCTATCTGCGGTTGCTGCGCCCACCTTTG |
| Rcn2(AxA)F | CCAAAAAGTCCAAGCGCAACGGCAAACGAGTTTTTCCAT |
| Rcn2(AxA)R | ATGGAAAAACTCGTTTGCCGTTGCGCTTGGACTTTTTGG |
| RCAN1(AxA)F | GACACCACTCCCAGCGCAGTGGCACATGTATGTGAGAGT |
| RCAN1(AxA)R | ACTCTCACATACATGTGCCACTGCGCTGGGAGTGGTGTC |
| Rcn1(VIVIT)F | TTGAAATCAAAGGTGCCTGTTATTGTTATTACTAGATGTACCACGAAC |
| Rcn1(VIVIT)R | GTTCGTGGTACATCTAGTAATAACAATAACAGGCACCTTTGATTTCAA |
| Rcn2(VIVIT)F | CCTCCAAAAAGTCCAGTTATAGTTATTACTGAGTTTTTCCAT |
| Rcn2(VIVIT)R | ATGGAAAAACTCAGTAATAACTATAACTGGACTTTTTGGAGG |
| RCAN1(VIVIT)F | GCGACTGACACCACTCCCGTTATTGTGATTACTGTATGTGAGAGTGAT |
| RCAN1(VIVIT)R | ATCACTCTCACATACAGTAATCACAATAACGGGAGTGGTGTCAGTCGC |
| Rcn1(ExxP-ala)F | TTCGATTTCAGCAAATGTGCAGCAGCGGCACAAAGGCATATACAAAGT |
| Rcn1(ExxP-ala)R | ACTTTGTATATGCCTTTGTGCCGCTGCTGCACATTTGCTGAAATCGAA |
| RCAN1(ExxP-ala)F | GTGGGATGGAAACAAGTGGCAGCTGCGGCCGCAGTCATAAACTATGATCTC |
| RCAN1(ExxP-ala)R | GAGATCATAGTTTATGACTGCGGCCGCAGCTGCCACTTGTTTCCATCCCAC |
| Rcn1(LxxP-ala)F | GAATTTAACTAAACAATACGCGAAGGTAGCAGAGAGTGAAAAAATGTTCC |
| Rcn1(LxxP-ala)R | GGAACATTTTTTCACTCTCTGCTACCTTCGCGTATTGTTTAGTTAAATTC |
| RCAN1(LxxP-ala)F | CATAGGAAGCTCACACGCGGCTCCGGCAAATCCAGACAAGCAGT |
| RCAN1(LxxP-ala)R | ACTGCTTGTCTGGATTTGCCGGAGCCGCGTGTGAGCTTCCTATG |
a
Cleavage sites for resrtiction endonucleases are underlined. Altered codon sequences used in site-directed mutagenesis are in boldface.