FIG. 4.

Growth of heterotopically injected B16F0 melanoma cells in Adam9^−/− mice and wild-type controls. (A). Relative weights of tumors developing from 10⁶ B16F0 melanoma cells injected into the flanks of Adam9^−/− (9−/−) mice compared to the average of tumor weights in wild-type (WT) control mice that were injected at the same time with the same culture of cells. The data are combined from three separate experiments (n = 10 for wild-type mice and n = 10 for Adam9^−/− mice in experiments 1 and 2, and n = 9 for wild-type mice and n = 9 for Adam9^−/− mice in experiment 3). Since the tumor sizes in wild-type mice at the end of each experiment varied somewhat, the average tumor size in wild-type mice in each experiment was set to 1, and the tumor size of individual wild-type mice and Adam9^−/− mice was calculated as a ratio to the average tumor size in wild-type mice. The reduction in tumor size in Adam9^−/− mice compared to that in wild-type controls was 54.5% (P < 0.0001). (B) Representative sections of tumors from wild-type (wt) or Adam9^−/− mice stained with anti-CD31 to mark endothelial cells. No difference in the overall distribution of larger vessels or capillaries was seen in tumors from wild-type or Adam9^−/− mice.