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. 2009 Mar 16;29(10):2865–2875. doi: 10.1128/MCB.01537-08

FIG. 6.

FIG. 6.

Kinetic analysis of ATP hydrolysis in anti-eIF4B immunoprecipitates. (A) eIF4B-associated activities from S6k1+/+ S6k2+/+ MEFs that were either not treated (gray squares) or treated with IFN-α for 90 min (black circles) are shown. (B) eIF4B-associated activities from S6k1−/− S6k2−/− MEFs that were either not treated (gray squares) or treated with IFN-α for 90 min (black circles) are shown. Data shown in panels A and B represent means ± standard errors of the results of five independent experiments. (C) eIF4B-associated activities from S6k1+/+ S6k2+/+ MEFs transfected with control siRNA that were either not treated (gray squares) or treated with IFN-α for 90 min (black circles) are shown. (D) eIF4B-associated activities from S6k1+/+ S6k2+/+ MEFs transfected with p170/eIF3A siRNA that were either not treated (gray squares) or treated with IFN-α for 90 min (black circles) are shown. Data shown in panels C and D represent means ± standard errors of the results of four independent experiments. V, velocity; wt, wild-type.