FIG. 1.

Sensitivity to rapamycin and caffeine of the diverse yeast type 2C protein phosphatase mutants. (A) Wild-type strain BY4741 and the different ptc mutants were spotted onto YPD plates containing the indicated concentrations of rapamycin or caffeine. Growth was monitored after 60 h of incubation at 28°C. (B) The BY4741 strain and its ptc1 derivative were transformed with an empty centromeric YCplac33 vector (YCp-Ø) or with the wild type or a catalytically inactive form of Ptc1 (PTC1^[D58N]) cloned in either centromeric (YCp) or high-copy-number (YEp) vectors. Cells were spotted as described above and incubated for 48 h.