FIG. 4.

Fli-1 knockdown reduces the proliferation of NN10 cells independently of cell death induction. Clone bcl2 22 was derived from NN10/TR/shfli_749#5 by transfection with a human Bcl2 expression vector. NN10/TR/shfli_749#5 and bcl2 22 cells were grown for 5 days with or without Dox before analysis. (A) Western blot analysis of the Fli-1, human Bcl2 (hBcl2), and murine Bcl2 (mBcl2) proteins. An unspecific band revealed by the Bcl2 antibody and which comigrates with hBcl2 is indicated by an asterisk. β-Actin revealed on the same membranes is shown as a loading control. (B) Relative cell growth, expressed as the percentages of total cells (viable and dead cells) in the presence of Dox (black bars) and in its the absence (white bars). (C) Percentages of dead cells determined by trypan blue in the presence (black bars) or absence (white bars) of Dox, respectively. (Means and standard deviations from three independent experiments are shown). Significant (P < 0.05) and nonsignificant differences are indicated by asterisks and NS, respectively.