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. 2009 Mar 16;29(10):2852–2864. doi: 10.1128/MCB.01435-08

FIG. 9.

FIG. 9.

Spi-1 and Fli-1 directly activate a common set of genes involved in ribosome biogenesis in 745A cells. 745A/TR and 745A/TR/shfli1_749#44 cells were treated with Dox or left untreated for 2 days, and differentiation was induced by adding 5 mM HMBA for 1 day before performing quantitative RT-PCR analyses of the indicated gene transcripts (45S corresponds to 45S rRNA precursor). (A) Relative levels of transcripts determined in 745A/TR cells (means and standard deviations from three independent experiments). (B) Same as panel A but for 745A/TR/shfli1_749#44 cells. (C) Spi-1 chromatin occupancy of the indicated gene promoters determined by chromatin immunoprecipitation on untreated 745A/TR/shfli1_749#44 cells using Spi-1-specific or control Ubc9 antibody. Gapdh promoter and α-globin gene 129k and 153k regions (2) were used as negative controls. Results are expressed as relative proportions of the input chromatin which has been precipitated by antibodies standardized to the background levels determined on the Gapdh gene promoter (means and standard deviations from three independent experiments). (D) Fli-1 chromatin occupancy of the indicated gene promoters, determined as in panel C by chromatin immunoprecipitation on untreated 745A/TR/shfli1_749#44 cells using Fli-1 antibody.