TABLE 1.
Phenotype induced by Fli-1 knockdown in NN10 cellsa
| shRNA used | Cell clone | Relative no. of cells, day 4
|
% Dead cells, day 3
|
% Benzidine-positive cells, day 5
|
Fold change in mRNA (+Dox/−Dox), day 2
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| −Dox | +Dox | −Dox | +Dox | −Dox | +Dox | Bcl2 | Alas2 | Ahsp | α-globin | ||
| None | TR | 100 | 96.4 (17.6) | <3 | <3 | 0 | 0 | 1.16 (0.12) | 1.2 | 1.4 | 0.9 (0.78) |
| SCR | 3B10 | 100 | 110 | <3 | <3 | 0 | 0 | ND | ND | ND | ND |
| 3E9 | 100 | 87 | <3 | <3 | 0 | 0 | ND | ND | ND | ND | |
| 1C6 | 100 | 98 | <3 | <3 | 0 | 0 | ND | ND | ND | ND | |
| Fli1_749 | 5 | 100 | 30.8 (13.4) | 1 (1.1) | 13.2 (7.3) | 0 | 6.4 (5.3) | 0.34 (0.22) | 3.2 (0.4) | 2.7 (0.6) | 12.5 (5) |
| 10 | 100 | 61 | <3 | 15.7 | 0 | 4.8 | ND | ND | ND | ND | |
| 23 | 100 | 73 | <3 | 11.8 | 0 | 2.8 | ND | ND | ND | ND | |
a
Means and standard deviations (in parentheses) from three independent experiments. ND, not determined; −Dox, no treatment; +Dox, Dox treatment. Statistically significant Dox effects are indicated in bold.