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. 2009 Mar 9;29(10):2532–2545. doi: 10.1128/MCB.01682-08

FIG. 2.

FIG. 2.

Sir3 occupancy at silent regions increases in the presence of the H3 D77N mutant. (A) Anti-Sir3 antibodies were used in ChIP experiments to determine localization of Sir3 at HMR E and HMR-a1 (histograms). A schematic representation of the HMR locus and the regions analyzed is shown. The bar graphs show average Sir3 occupancy with vector, wild-type Sir3, or Sir3 A2G plasmids in strains with wild-type H3 (VSY29), H3 D77N (VSY30), or H4 H75Y (VSY31). (B) A schematic representation of TEL-VIR and the regions analyzed is shown. Sir3 occupancy at 0.67 kb and 5 kb from the telomere is presented in the bar graphs. Strains used were the same as for the experiment whose results are shown in panel A. The data shown are the averages and standard deviations of the results of two PCR amplifications of one representative IP. Sir3 localization in the presence of wild-type histone H3, the histone H3 D77N mutant, or the histone H4 H75Y mutant is depicted. WT, wild type.