FIG. 2.

Leakage of vessels was detected by EB in the brains of rats infected 14 days previously with PVC-211 MuLV. EB was injected into rats treated 7 to 24 days previously with PVC-211 MuLV or medium. Three rats were used for each group, and each experiment was repeated two times, for a total of nine control and nine virus-infected rats. (A) EB was extracted by formamide from whole brains and livers and the levels measured as described in Materials and Methods. A representative experiment is shown. Data are means ± standard errors of the means. (B) Sections of brain stem (a to c) or cerebellum (d) from control rats (a) or PVC-211 MuLV-infected rats (b to d) 14 dpi were examined for EB fluorescence (red) under a fluorescent microscope. The samples used for panels b to d are from different PVC-211 MuLV-infected animals. BV, blood vessel. Nuclei are counterstained with DAPI (blue). Magnification, ×20.