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. 2009 Mar 4;83(10):4952–4962. doi: 10.1128/JVI.00170-09

FIG. 2.

FIG. 2.

BGLF5 is required for virus production but not B-cell immortalization. (A) qPCR analysis with supernatants from lytically induced 293/ΔBGLF5, 293/ΔBGLF5-C and 293/EBV-wt cells. The concentration of viral geq is reduced in ΔBGLF5 supernatants compared to EBV-wt and ΔBGLF5-C supernatants. Presented are the mean values from eight independent experiments. (B) Electron micrographs of pelleted ΔBGLF5 and ΔBGLF5-C virus supernatants. ΔBGLF5 supernatants contained a lower number of mature virions in comparison with ΔBGLF5-C supernatants. Arrows indicate mature virions. Bar, 0.2 μm. (C) Raji B-cell infection assay. The concentration of infectious particles in ΔBGLF5 supernatants (as measured by green Raji cells) is reduced compared to EBV-wt and ΔBGLF5-C supernatants (mean value from eight independent experiments). (D) Multiple infections were carried out with 293 cells as target cells. Mean values of the percentages of infected cells are given. (E) Immortalization experiments with primary B cells from different donors. The results are given as mean values of the percentage of wells with cell outgrowth.