FIG. 2.

Immunoprecipitation of E^rns proteins from different pestiviruses. Proteins were transiently expressed in BHK-21 cells and metabolically labeled, and the cells were lysed with a lysis buffer containing 1% Triton X-100. Proteins were precipitated with an E^rns-specific serum and the precipitates separated on SDS-PAGE under nonreducing conditions. The transfected plasmids are indicated above the lanes: pBN^ATCC-E^rns-171C and pBN^ATCC-E^rns-171R, E^rns sequences from variants of BVDV NADL (ATCC VR534); pBN^MC-E^rns, E^rns sequence derived from biologically cloned BVDV NADL (5); pC-E^rns, E^rns from CSFV Alfort/Tübingen, wt or with the indicated mutations. For the other constructs, see the legend to Fig. 1 and the text. Broad bands or double bands (pB-E^rns-V5 and pB-E^rns-V5^C171R) are due to variations in the native glycosylation of the proteins.