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Determination of RNase activity present in crude extracts of SK6 cells infected with wt CSFV Alfort/Tübingen or the recombinant virus V-C171F (dimerization negative), V-C171d (dimerization negative), or V-H297K (RNase negative). Noninfected SK6 cells served as a negative control (mock). The enzymatic degradation of poly(U) was determined by measuring the optical density at 260 nm (OD260) as a marker of the release of small acid-soluble RNA fragments (27, 47).
