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Growth curve of the recombinant CSFV mutant V-C171S (dimerization negative) compared with those of the wt virus Alfort/Tübingen (wt) and mutant V-H297K (RNase negative). SK6 cells were infected with the CSFV variants at an MOI of 0.1 and harvested by freezing and thawing at the indicated time points. Titers were determined by immunofluorescence staining with monoclonal antibody a18 at 72 h p.i. The curves represent the mean values from two individual experiments.
